It has been shown recently that the free Ca2+ content of human red cel
ls rises during ageing in vivo. With the aim of determining the mechan
isms involved in such a change, we have investigated some aspects of C
a2+ homeostasis. Both the initial rate of Ca2+ influx and some kinetic
parameters of the Ca2+ pump of human red cells were studied in light
and dense sub-populations obtained through stringent, self-formed Perc
oll gradients. At 37 degrees C and pH 7.4, no differences in Ca2+ entr
y were found. By contrast, either at pH 7.0 or 7.4, the maximal Ca2+ e
xtrusion rate of the approximately 10% heaviest cells was one-half of
the corresponding lighter ones. The results demonstrate that the eleva
ted free Ca2+ concentration distinctive of senescent cells, arises fro
m a reduction in Ca2+ extrusion capacity during ageing. The possible p
hysiological significance of this finding is discussed.