IDENTIFICATION AND CHARACTERIZATION OF (GA CT)(N)-MICROSATELLITE LOCIFROM QUERCUS-PETRAEA/

Authors
STEINKELLNER H FLUCH S TURETSCHEK E LEXER C STREIFF R KREMER A BURG K GLOSSL J
Citation
H. Steinkellner et al., IDENTIFICATION AND CHARACTERIZATION OF (GA CT)(N)-MICROSATELLITE LOCIFROM QUERCUS-PETRAEA/, Plant molecular biology, 33(6), 1997, pp. 1093-1096
Citations number
24
Categorie Soggetti
Plant Sciences",Biology
Journal title
Plant molecular biology → ACNP
ISSN journal
01674412
Volume
33
Issue
6
Year of publication
1997
Pages
1093 - 1096
Database
ISI
SICI code
0167-4412(1997)33:6<1093:IACO(C>2.0.ZU;2-Y
Abstract
In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)(n)-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in e. p etraea and Q. robur. 17 out Of 52 tested primer pairs resulted in the amplification of a polymorphic single-locus pattern. The number of all eles found per locus varied from 6 to 16. Combining the genetic variat ion observed for the characterized loci provides a unique genotype for all the individuals tested. Using intraspecific controlled crosses of e. robur trees Mendelian inheritance could be shown for five loci.