IDENTIFICATION OF A HUMAN GENE ENCODING A HOMOLOG OF SACCHAROMYCES-CEREVISIAE EXO1, AN EXONUCLEASE IMPLICATED IN MISMATCH REPAIR AND RECOMBINATION

The EXO1 gene was identified in Saccharomyces cerevisiae as a gene enc oding an exonuclease that interacts with MSH2 and functions in mismatc h repair and genetic recombination, To understand the role of EXO1 in higher eukaryotes, we identified the human EXO1 gene. The hEXO1 predic ted amino acid sequence shares 26.6% identity with the S. cerevisiae E XO1 amino acid sequence. The human and S, cerevisiae proteins showed a similar ability to complement the mutator phenotype of S. cerevisiae rad27 mutants indicating that the two proteins are functionally simila r. There appear to be two forms of hEXO1 that differ by the COOH-termi nal 1 and 44 amino acids, respectively, and these appear to result fro m alternative RNA splicing. The hEXO1 gene consists of 14 exons and is transcribed to yield a 3-kb mRNA, Radiation hybrid and fluorescence i n situ hybridization mapping studies indicate that the human gene is l ocated at 1q42.2-qter. Northern blot analysis demonstrates that hEXO1 is expressed in high levels in testis; elevated expression was also ob served in thymus and colon and to a lesser extent in small intestine, placenta, spleen, and ovary.