VECTORS FOR THE EXPRESSION OF TAGGED PROTEINS IN SCHIZOSACCHAROMYCES-POMBE

A series of vectors is described which enables the episomal expression of proteins fused to different tag sequences in Schizosaccharomyces p ombe. Proteins can be expressed with their amino termini fused to GFP/ EGFP, three copies of the HA or Pk epitopes or a combined tag which co ntains two copies of the myc epitope and six histidine residues (MH). Fusion of the carboxyl terminus of a protein to a tag is possible with GFP/EGFP or Pk. Expression of the fusion proteins is controlled by th e medium strength mutant version of the regulatable nmt1 promoter. (C) 1998 Elsevier Science B.V. All rights reserved.