PREDICTIVE VALUE OF VIRAL LOAD AND OTHER MARKERS FOR PROGRESSION TO CLINICAL AIDS AFTER CD4+ CELL COUNT FALLS BELOW 200 MU-L/

Background To assess the predictive value of biological and clinical e vents for progression to AIDS (1993 European classification) when the CD4+ cell count falls below 200/mu l (CD4 threshold) in different expo sure groups. To investigate whether such markers remain predictive ind ependently of the serum HIV-1 RNA level at the CD4 threshold. Methods The predictive value of biological and clinical events occurring durin g the 24 months prior to the occurrence of CD4 threshold (n = 333) was quantified in a Cox model. Another Cox model was carried out in a sub set of 77 patients in whom viral load from stored sera was available. Furthermore, changes in viral load during the 24 months preceding the CD4 threshold were assessed in a mixed model according to subsequent d evelopment of AIDS. Results Among the 333 patients, the slope of the C D4+ cell counts, the emergence of p24 antigen, persistent thrush, and age at the CD4 threshold were independent predictors of progression to clinical AIDS (44.7%). Among the subset of 77 patients, the HIV-I RNA level at the CD4 threshold, persistent thrush and age remained indepe ndent predictors of progression to AIDS (45.5%). The increase of the H IV-I RNA level was moderate, both in non-progressors (24.0% per year) and in those who subsequently developed AIDS (27.1% per year), (P = 0. 93). Viral load was consistently higher in the latter group (P = 0.002 ). Conclusion At a late stage of infection, age and persistent thrush remain predictive of progression to AIDS, independently of viral load.