IMPROVED HUMAN ZYGOTE DEVELOPMENT IN A MODIFIED HAMS F1O MEDIUM IN-VITRO

Propose: Our purpose was to determine the effect of modified Ham's F10 media with and without glucose, hypoxanthine, phosphate, and transiti on metals (MM) on human embryo development. Methods: Patients with at least one zygote were randomized by alternate assignment to one of the treatment groups. The treatment groups were as follows: normal Hams F 10, MM, and MM with 0.5 mM glucose (LGMM). Patients were undergoing in vitro fertilization and embryo transfer or frozen-thawed embryo trans fer: Zygotes (fresh and frozen-thawed) were incubated in one of three media for 24 hr before transfer. The groups were compared with respect to embryo quality, number of embryos transferred age of the patients, clinical pregnancy rate, and implantation rate. Results: The clinical pregnancy rate and the implantation rate were significantly higher in the group of patients whose zygotes were cultured in MM. LGMM did not appear to improve the pregnancy outcome compared to normal Ham's F10. However the quality of the embryos cultured in MM or LGMM appears to be superior to the normal Ham's F10 with respect to the number of exce llent and good-quality embryos and embryos without fragments. Conclusi on: A modified Ham's F10 medium (MM) without added glucose appears to be superior to normal Ham's F10 for culture of human zygotes for embry o transfer.