Recent focus has been placed on ecoregion delineations for providing a
n appropriate framework for monitoring and assessment of region-specif
ic attainable water/habitat quality. Using an ecoregion approach to st
ratify variance, this study was conducted to determine whether earlier
(subjectively) selected Swedish reference lakes may be considered as
regionally representative reference sites when compared with a randoml
y selected lake population. Predictive modeling by discriminant functi
on analysis with lakes classified by ecoregion and lake surface area a
nd six physicochemical variables showed that the greater majority of r
eference lakes may be considered as regionally representative. The hig
hest proportion of lake ''misclassifications'' occurred in the boreone
moral ecoregion, a relatively diverse ecoregion of southern Sweden. Th
is apparent bias may be in part be due to the criteria used in selecti
ng regional reference lakes. In the earlier selection of reference lak
es emphasis was placed on lakes not being adversely affected by land u
sage or pollutant discharges, consequently forest lakes were often ove
rrepresented and sites in agricultural areas underrepresented in the s
elected reference sites. As a complement to predictive modeling, PCA o
rdination showed the placement of reference lakes within the random la
ke population and indicated where reference sites might be missing alo
ng potentially important ecological gradients.