EFFECT OF BOVINE KAPPA-CASEINOGLYCOPEPTIDE ON SECRETION OF INTERLEUKIN-1 FAMILY CYTOKINES BY P388D1 CELLS, A LINE DERIVED FROM MOUSE MONOCYTE MACROPHAGE/
M. Monnai et H. Otani, EFFECT OF BOVINE KAPPA-CASEINOGLYCOPEPTIDE ON SECRETION OF INTERLEUKIN-1 FAMILY CYTOKINES BY P388D1 CELLS, A LINE DERIVED FROM MOUSE MONOCYTE MACROPHAGE/, Milchwissenschaft, 52(4), 1997, pp. 192-196
P388D1 cells, a line derived from DBA/2 mouse monocyte/macrophage, pro
duced an inhibitory factor, which was neutralized by goat anti-IL-1ra
polyclonal antibody, by lipopolysaccharide(LPS)-induced proliferation
of mouse spleen cells in the presence of kappa-caseinoglycopeptide (re
sidues 106-169 of kappa-casein, CGP) but not in the absence of CGP. Re
verse transcription-polymerase chain reaction analysis showed that P38
8D1 cells transcribed IL-1ra mRNA in both the absence and the presence
of CGP. These observations indicate that CGP might increase IL-1ra bi
osynthesis and/or secretion. On the other hand, CGP did not affect sec
retion of IL-1 alpha and IL-1 beta produced from P388D1 cells in eithe
r the absence or presence of LPS. Moreover, little IL-1ra was secreted
when CGP was added after 1 h cultivation of P388D1 cells with LPS, Th
ese results suggest that CGP is not able to induce IL-1ra production f
rom previously activated macrophages. CGP may contribute to antiinflam
matory response in neonatal intestine by induction of IL-1ra produced
from monocytes/macrophages of maternal milk origin.