HIGH-RESOLUTION GENETIC, PHYSICAL, AND TRANSCRIPT MAP OF THE MND2 REGION OF MOUSE-CHROMOSOME-6

The autosomal recessive mutation mnd2 is responsible for a lethal neur omuscular wasting disorder in the mouse. A high-resolution genetic map of the mnd2 region of mouse chromosome 6 was generated by analysis of 1147 F2 offspring from an intersubspecific cross between strains C57B L/6J-mnd2/+ and CAST/Ei. The results localize mnd2 to the 0.2-cM inter val between D6Mit164 and D6Mit128. A contig of overlapping YAC, BAG, a nd pi clones spanning the nonrecombinant interval was constructed. One novel gene isolated from the contig, D6Mm3e, is a new member of the W D repeat gene family, The observed gene order for the five positional candidate genes previously mapped to the region and five newly isolate d genes is centromere-Hexokinase D6Mm5e-p62 Dok-Aup1-Rhotekin, D6Mm3e- Dynactin 1-Smooth muscle gamma actin-D6Mm4e-beta-adducin-telomere. Sev en of these genes are located within the 400-kb nonrecombinant interva l for mnd2. Comparison between wildtype and mutant failed to detect an y differences in mRNA size, abundance, or coding sequence for these se ven genes. The genes described here are positional candidates for the Parkinson disease susceptibility locus PARK3 that was recently mapped to the corresponding region of human chromosome band 2p13.1, (C) 1998 Academic Press.