K. Tokimoto et al., SELECTION OF SHIITAKE STRAINS GREATLY COMPETITIVE TO ANTAGONISTIC FUNGI TRICHODERMA SPP, Mokuzai Gakkaishi, 44(5), 1998, pp. 351-359
Trichoderma species cause a large crop loss in the log cultivation of
shiitake mushroom, Lentinula edodes (Berk.) Pegler, thus new shiitake
strains greatly competitive with the Trichoderma species are required
for their successful production. We examined the competitive ability o
f L. edodes against Trichoderma attack mainly using the dual culture o
n sawdust media to establish a useful selection method for L. edodes s
trains which have great competitive abilities. The degree of L. edodes
competitive ability against Trichoderma attack varied depending on th
e difference of qualities and quantities of nutritional components sup
plemented into the sawdust medium, and the sawdust medium mixed with 1
/3 volume of rice bran was useful in estimating the competitive abilit
ies of dikaryotic strains of L. edodes. Further more, greatly competit
ive Lentinula dikaryons selected by the dual culture appeared to be ab
le to repel Trichoderma attack in outdoor log cultivation. The test us
ing sawdust medium with 1/3 volume of rice bran revealed that the comp
etitive abilities of dikaryons did not correlate with the competitive
ability of their parental monokaryons. However, selection of monokaryo
ns which produced greatly competitive dikaryons was possible: each mon
okaryon was crossed with several test monokaryons, and the mean of the
competitive abilities of the resultant dikaryons was determined.