Fourteen different starter cultures containing one strain of Saccharom
yces cerevisiae with and without individual or combinations of lactic
acid bacteria (Lactobacillus plantarum, Lact. sanfrancisco, Enterococc
us faecium, Leuconostoc mesenteroides) were employed to investigate th
e role of leavening microflora on the properties of pizza doughs. Micr
obiological, chemical and physical characteristics of doughs prepared
with the same flour and under the same processing conditions were dete
rmined. Leavening times and acidification properties depended on the m
icrobial association used. The proportions of lactic and acetic acid p
roduced by lactic acid bacteria were consistent with the metabolic pro
perties of the strains employed. The bacteria/yeast ratios arising fro
m microbial counts at the end of the leavening process were always lon
-er in comparison to sour- or bread-doughs. The size of the yeast popu
lation did not change much, while bacteria showed from one to four dup
lications. Rheologically, the fermented doughs could only be significa
ntly distinguished from the control dough with regard to the elastic m
odulus. Principal Component Analysis was applied to the acidimetric da
ta. The scattergram of the two principal components effectively discri
minated 13 of the 14 pizza dough types.