THE EFFECT OF PH ON THE FUNCTIONAL-PROPER TIES OF M-4-LACTATE DEHYDROGENASE FROM SKELETAL-MUSCLES OF FISH AT THERMAL ADAPTATIONS

Authors
KLYACHKO OS POLOSUKHINA ES KLYACHKO VA OZERNYUK ND
Citation
Os. Klyachko et al., THE EFFECT OF PH ON THE FUNCTIONAL-PROPER TIES OF M-4-LACTATE DEHYDROGENASE FROM SKELETAL-MUSCLES OF FISH AT THERMAL ADAPTATIONS, Biofizika, 42(2), 1997, pp. 334-337
Citations number
19
Categorie Soggetti
Biophysics
Journal title
Biofizika → ACNP
ISSN journal
00063029
Volume
42
Issue
2
Year of publication
1997
Pages
334 - 337
Database
ISI
SICI code
0006-3029(1997)42:2<334:TEOPOT>2.0.ZU;2-M
Abstract
The effect of pH on the activity of M-4-lactate dehydrogenase purified from skeletal muscles of loach acclimated over 25 days to 5 degrees C (<<cold>> enzyme) and to 18 degrees C (<<warm>> enzyme) was studied. The pH optima for the two forms of the enzyme coincide. However, in th e pH range 5,0-6,5, the activity of <<cold>> enzyme shows greater stab ility. This conclusion is confirmed by the kinetical studies of pH inf luence on the activity of two forms of lactate dehydrogenase. The spec ific activity of <<cold>> enzyme is higher than the <<warm>> one.