EXPRESSION OF BACTERIAL CHORISMATE PYRUVATE-LYASE IN TOBACCO - EVIDENCE FOR THE PRESENCE OF CHORISMATE IN THE PLANT CYTOSOL

The bacterial gene ubiC encodes chorismate pyruvatelyase, an enzyme wh ich converts chorismate to 4-hydroxybenzoate and which is not normally present in plants. The UbiC protein was expressed in tobacco, with ta rgeting of the gene product either to the plastids or to the cytosol. In both cases, chorismate pyruvate-lyase activity and a resulting form ation of 4-hydroxybenzoate was detected. This suggests that chorismate , a metabolite of the shikimate pathway, is present not only in the pl astids but also in the cytosol of plant cells.