CAPILLARY ELECTROPHORESIS OF CYTOKININS AND CYTOKININ RIBOSIDES

Citation
V. Pacakova et al., CAPILLARY ELECTROPHORESIS OF CYTOKININS AND CYTOKININ RIBOSIDES, Journal of chromatography, 764(2), 1997, pp. 331-335
Citations number
26
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Volume
764
Issue
2
Year of publication
1997
Pages
331 - 335
Database
ISI
SICI code
Abstract
A CE separation of cytokinins and cytokinin ribosides and some other p urine and pyrimidine bases has been developed. Two electrolyte systems have been tested: 150 mM phosphoric acid, pH 1.8 and 50 mM sodium dod ecylsulphate + 20 mM berate, pH 9.2. The migration times were measured and the effects of the solute structures were discussed. Preliminary experiments with plant extracts have been performed to identify the cy tokinins and their ribosides. Both the systems can be used, but 150 mM phosphoric acid is better suited for identification of cytokinins in plant extracts, as the electropherograms are subject to fewer interfer ences.