BIOSYNTHETIC-STUDIES OF FUMONISIN B-1 AND AAL TOXINS

The biosynthesis of the sphinganine analogue mycotoxins (SAMs) fumonis in B-1 and the AAL toxins was studied by growing Fusarium moniliforme and Alternaria alternata f. sp. lycopersici in liquid culture. Radioac tive and stable isotopically labeled amino acid, water, and molecular oxygen precursors were added to the culture media and toxins were anal yzed using thin-layer chromatography, liquid scintillation counting, h igh-performance liquid chromatography (HPLC), C-13 nuclear magnetic re sonance (NMR) spectroscopy, and electrospray mass spectrometry (ESMS). Results indicated that glycine was incorporated directly into the AAL toxins and that methionine was incorporated into the AAL toxin methyl groups. Oxygens in tricarballylic acid moieties for fumonisin B1 and the AAL toxins were derived from H2O while the lipid backbone hydroxyl s for fumonisin BL and the AAL toxins originated from molecular oxygen . Isotopic enrichment patterns for the various AAL toxins showed marke d differences among toxins, suggesting a complex, rather than sequenti al, biosynthetic pattern.