This study compares the performances of animal-based byproducts with o
ther complex nutrients. Productions of alpha-amylase by Bacillus subti
lis, glucoamylase by Aspergillus niger, endocellulase, exocellulase, a
nd xylanase by Trichoderma viride, protease by B. subtilis, and B. lic
heniformis were evaluated in shake-flask fermentation. Culture broths
were analyzed daily for enzyme activity for up to 9 days. Significant
increases in a-amylase, cellulase, and xylanase activities were observ
ed. Data strongly suggest that many of these byproducts can be used to
replace the more expensive complex nutrients in industrial enzyme pro
duction with a corresponding increase in enzyme production.