MEDIA EVALUATION FOR THE PRODUCTION OF MICROBIAL ENZYMES

This study compares the performances of animal-based byproducts with o ther complex nutrients. Productions of alpha-amylase by Bacillus subti lis, glucoamylase by Aspergillus niger, endocellulase, exocellulase, a nd xylanase by Trichoderma viride, protease by B. subtilis, and B. lic heniformis were evaluated in shake-flask fermentation. Culture broths were analyzed daily for enzyme activity for up to 9 days. Significant increases in a-amylase, cellulase, and xylanase activities were observ ed. Data strongly suggest that many of these byproducts can be used to replace the more expensive complex nutrients in industrial enzyme pro duction with a corresponding increase in enzyme production.