EVALUATION OF THE ABBOTT LCX ASSAY FOR DETECTION OF NEISSERIA-GONORRHOEAE IN ENDOCERVICAL SWAB SPECIMENS FROM FEMALES

The Abbott LCx Neisseria gonorrhoeae assay (Abbott Laboratories, Abbot t Park, ill.) uses a ligase chain reaction (LCR) amplification in the LCx probe system for detection of a specific nucleotide sequence in th e Opa-encoding gene of N. gonorrhoeae. We evaluated the LCx assay in a comparison with conventional culture employing modified Thayer-Martin media for the detection of N. gonorrhoeae from female endocervical sp ecimens obtained from patients attending a sexually transmitted diseas e clinic. Discordantly LCR-positive and culture-negative specimens wer e further evaluated by testing with another LCR assay which used an N, gonorrhoeae-specific pilin probe. Specimens positive by both LCR assa ys were considered confirmed LCx-positive specimens. A specimen was co nsidered to contain N, gonorrhoeae when it was either culture positive or culture negative and confirmed LCx positive. A total of 403 female endocervical specimens were evaluated. The prevalence of N. gonorrhoe ae in this population was 8.7%. The sensitivity and specificity of the LCx assay were 94.3 and 99.4%, and those of culture were 77.1 and 100 %, respectively. The Abbott LCx assay is a rapid, sensitive method for detection of N, gonorrhoeae in female endocervical specimens.