RAPID PCR DETECTION OF HELICOBACTER-PYLORI-ASSOCIATED VIRULENCE AND RESISTANCE GENES DIRECTLY FROM GASTRIC BIOPSY MATERIAL

Authors
BJORKHOLM B BEFRITS R JAUP B ENGSTRAND L
Citation
B. Bjorkholm et al., RAPID PCR DETECTION OF HELICOBACTER-PYLORI-ASSOCIATED VIRULENCE AND RESISTANCE GENES DIRECTLY FROM GASTRIC BIOPSY MATERIAL, Journal of clinical microbiology (Print), 36(12), 1998, pp. 3689-3690
Citations number
19
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology (Print) → ACNP
ISSN journal
00951137
Volume
36
Issue
12
Year of publication
1998
Pages
3689 - 3690
Database
ISI
SICI code
0095-1137(1998)36:12<3689:RPDOHV>2.0.ZU;2-Z
Abstract
We have developed a PCR-based method to detect macrolide resistance an d the virulence gene cagA in Helicobacter pylori within 24 h, thereby improving the lengthy process of culture-based approaches. Total DNA w as prepared directly from stomach biopsy specimens. The procedure prov ed to be rapid and reliable and could be utilized for diagnostic purpo ses.