A 'simulated fluorescence' three-dimensional reconstruction algorithm,
which is especially suitable for confocal images of partial transpare
nt biological samples, is proposed in this paper. To make the retina p
rojection of the object reappear and to avoid excessive memory consump
tion, the original image is rotated and compressed before the processi
ng. A left image and a right image are mixed by different colors to in
crease the sense of stereo. The details originally hidden in deep laye
rs are well exhibited with the aid of an 'auxiliary directional source
'. In addition, the time consumption is greatly reduced compared with
conventional methods such as 'ray tracing'. The realization of the alg
orithm is interpreted by a group of reconstructed images. (C) 1998 Els
evier Science Ltd. All rights reserved.