MACROCYCLIC CU(II)TETRAANHYDROAMINOBENZALDEHYDE COMPLEX ANTIPROLIFERATIVE ACTIVITY IN-VITRO AND IN-VIVO

The macrocyclic Cu(II)-tetraanhydroaminobenzaldehyde complex Cu(TAAB)C 12 induces various cytotoxic effects in the dependence on a cell line, concentration and time of exposition. The highest complex concentrati on of 914 nmol/l induces degeneration of certain part of the HeLa cell s population after 24 hours of cultivation. The concentration of 183 n mol/l causes a delayed cytotoxic effect on HeLa and HepG2 cells. After 24 hours of culturing 15.4-28.4% of cell population proliferated but after 48 and 72 hours 2.0-42.3% of the cell population degenerated The cytotoxic effect on V79 cells is directly dependent on the actual con centration and time of the complex influence. The cytotoxic concentrat ions of Cu(TAAB)Cl-2 induce an integrity damage of cytoplasmatic membr ane and two phase unbalanced growth. Cu(TAAB)Cl-2 possesses an antileu kemic activity which at the dose of 10 mg/kg of body weight is not acc ompanied by side toxic effects on mice.