PRODUCTION OF ALL 8 GENOTYPES OF NULL ALLELES AT WAXY LOCI IN BREAD WHEAT, TRITICUM-AESTIVUM L

Null alleles of the three loci coding for 'waxy' proteins in bread whe at have been identified. Plants carrying different null alleles were c ollected and segregation of the null alleles in both selfed and double d haploid progeny of plants simultaneously heterozygous for the null a lleles at each of the three loci were examined by sodium dodecyl sulph ate-polyacrylamide gel electrophoresis (SDS-PAGE). Mendelian segregati on of these alleles in both types of progeny was shown, indicating tha t they can easily be utilized in breeding programmes. Iodine staining of the eight possible null phenotypes showed that only the triple null type had zero amylose in its endosperm starch.