K. Turgut et al., EFFICIENT SHOOT REGENERATION AND SOMATIC EMBRYOGENESIS FROM IMMATURE COTYLEDONS OF BRASSICA-NAPUS L, Plant breeding, 117(5), 1998, pp. 503-504
Immature cotyledons of Brassica napus cv. 'Topas' were used to investi
gate the effects of various naphthaleneacetic acid and benzylaminopuri
ne concentrations on morphogenesis in vitro. A high efficiency of adve
ntitious shoot regeneration and somatic embryogenesis was achieved on
Murashige and Skoog medium supplemented with the above growth regulato
rs. Two types of somatic embryogenesis were obtained, the first direct
ly and the second via a callus stage.