A NEW TECHNIQUE OF SCREENING GENE MUTATION - FLUORESCENT FINGERPRINTING

A new technique called fluorescent fingerprinting (FF) was set up and used to screen and detect gene mutation from a large number of samples by using fluorescent labelled ddNTP and ABI-373A sequencer. By compar ing the colour ladders of several lanes from the same sequencing regio n of different samples, any band with a different colour from its neig hbours or a pattern displacement could be easily found out in the Maci ntosh screen which indicates a gene mutation. Then, the sequencing pea k-chromatograms were made out only for the selected samples. (C) 1998 Elsevier Science B.V. All rights reserved.