The isolation of two oxidation products of hyperforin from the aerial
parts of Hypericum perforatum and their structure determination by mea
ns of 2D NMR methods is reported. The products had the same 1-(2-methy
l-1-oxopropyl)-2,12-dioxo-3,10 beta-bis(3-methyl-2-butenyl)-11 beta-me
thyl-11 3-pentenyl)-5-oxatricyclo[6.3.1.0(4.8)]-3-dodecene skeleton. I
n addition, one of them, with the same number of carbons as hyperforin
(C35H52O5), contained a 1-methyl-1-hydroxyethyl group in the 6 beta-p
osition, whereas the other compound (a hemiacetal, C32H46O5), presumab
ly a degradation product of hyperforin, exhibited a 6-hydroxy function
. The latter was an inseparable mixture of 6 alpha- and 6 beta-hydroxy
epimers undergoing (according to phase sensitive NOESY) mutual :inter
conversion. (C) 1998 Elsevier Science Ltd. All rights reserved.