CLONING AND FUNCTIONAL-CHARACTERIZATION OF THE MOUSE P2X(7) RECEPTOR

Authors
CHESSELL IP SIMON J HIBELL AD MICHEL AD BARNARD EA HUMPHREY PPA
Citation
Ip. Chessell et al., CLONING AND FUNCTIONAL-CHARACTERIZATION OF THE MOUSE P2X(7) RECEPTOR, FEBS letters, 439(1-2), 1998, pp. 26-30
Citations number
15
Categorie Soggetti
Biology,"Cell Biology",Biophysics
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
439
Issue
1-2
Year of publication
1998
Pages
26 - 30
Database
ISI
SICI code
0014-5793(1998)439:1-2<26:CAFOTM>2.0.ZU;2-J
Abstract
We have isolated a 1785-bp complementary DNA (cDNA) encoding the murin e P2X(7) receptor subunit from NTW8 mouse microglial cells. The encode d protein has 80% and 85% homology to the human and rat P2X(7) subunit s, respectively. Functional properties of the heterologously expressed murine P2X(7) homomeric receptor broadly resembled those of the P2X(7 ) receptor in the native cell line. However, marked phenotypic differe nces were observed between the mouse receptor, and the other P2X(7) re ceptor orthologues isolated with respect to agonist and antagonist pot encies, and the kinetics of formation of the large aqueous pore. (C) 1 998 Federation of European Biochemical Societies.