ADAPTIVE PROTECTION AGAINST THE INDUCTION OF OXIDATIVE DNA-DAMAGE AFTER HYPERBARIC-OXYGEN TREATMENT

Hyperbaric oxygen (HBO) treatment (i.e. exposure to 100% oxygen at a p ressure of 2.5 ATA for a total of three 20 min periods) of human subje cts caused clear and reproducible DNA effects in the comet assay with leukocytes. Interestingly, DNA damage was detected only after the firs t treatment and not after further treatments under the same conditions , indicating an increase in antioxidant defences. We now demonstrate t hat blood taken 24 h after HBO treatment is well protected against the in vitro induction of DNA damage by hydrogen peroxide (H2O2). H2O2 tr eatment caused a significant induction of DNA effects in the comet ass ay and chromosome breakage in the micronucleus test in the blood of vo lunteers before HBO. The same treatment did not cause genotoxic effect s 24 h after HBO, This protective effect lasted for at least 1 week. E xperiments with isolated lymphocytes gave similar results, indicating that the adaptive response is a cellular effect. The cells were not co mparably protected against the genotoxic effects of gamma-irradiation, suggesting increased scavenging of reactive oxygen species distant fr om nuclear DNA.