EXPRESSION OF A FUSION PROTEIN OF SCFV-BIOTIN MIMETIC PEPTIDE FOR IMMUNOASSAY

Authors
LUO D GENG M SCHULTES B MA J XU D HAMZA N QI W NOUJAIM A MADIYALAKAN R
Citation
D. Luo et al., EXPRESSION OF A FUSION PROTEIN OF SCFV-BIOTIN MIMETIC PEPTIDE FOR IMMUNOASSAY, Journal of biotechnology, 65(2-3), 1998, pp. 225-228
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Journal of biotechnology → ACNP
ISSN journal
01681656
Volume
65
Issue
2-3
Year of publication
1998
Pages
225 - 228
Database
ISI
SICI code
0168-1656(1998)65:2-3<225:EOAFPO>2.0.ZU;2-Z
Abstract
We constructed two fusion proteins of scFv linked to biotin mimetic se quence (BMS) via different linkers, and expressed them in the Pichia p astoris expression/secretion system. We found that both bi-functional scFv proteins exhibited their intrinsic binding activities to antigen CA125 determined in competitive radioimmunoassay experiments, bur the fusion protein with a spacer between the scFv and EMS (scFv-spacer-BMS ) showed higher binding activity of streptavidin than the one with c-M yc peptide as a linker. (C) 1998 Elsevier Science B.V. All rights rese rved.