WATER-SOLUBLE CONJUGATE OF DOUBLE-STRANDED DNA AND POLY(N-ISOPROPYLACRYLAMIDE) FOR ONE-POT AFFINITY PRECIPITATION SEPARATION OF DNA-BINDINGPROTEINS

Authors
UMENO D KAWASAKI M MAEDA M
Citation
D. Umeno et al., WATER-SOLUBLE CONJUGATE OF DOUBLE-STRANDED DNA AND POLY(N-ISOPROPYLACRYLAMIDE) FOR ONE-POT AFFINITY PRECIPITATION SEPARATION OF DNA-BINDINGPROTEINS, Bioconjugate chemistry, 9(6), 1998, pp. 719-724
Citations number
25
Categorie Soggetti
Chemistry Inorganic & Nuclear",Biology,"Biochemical Research Methods",Chemistry
Journal title
Bioconjugate chemistry → ACNP
ISSN journal
10431802
Volume
9
Issue
6
Year of publication
1998
Pages
719 - 724
Database
ISI
SICI code
1043-1802(1998)9:6<719:WCODDA>2.0.ZU;2-M
Abstract
Poly(N-isopropylacrylamide) having a terminal psoralen group was synth esized and covalently bound to plasmid pBR 322 DNA through a photochem ical reaction. The resulting conjugate exhibited temperature-responsiv e precipitation due to the aggregation of poly(N-isopropylacrylamide) chains when heated above 31 degrees C. This system was used for the on e-pot separation of restriction endonuclease EcoRI.