SYNTHESIS OF OLIGODEOXYNUCLEOTIDES CONTAINING N-4-MERCAPTOETHYLCYTOSINE AND THEIR USE IN THE PREPARATION OF OLIGONUCLEOTIDE-PEPTIDE CONJUGATES CARRYING C-MYC TAG-SEQUENCE

The preparation and properties of oligodeoxynucleotides containing mer captoethyl groups at position N-4 of cytosine are described. The resul ting thiol-oligodeoxynucleotides were reacted with a maleimido-peptide carrying the c-myc tag-sequence. The peptide-oligonucleotide conjugat e is specifically recognized by an anti c-myc monoclonal antibody, thu s constituting a labeling system with sensitivity similar to other exi sting methods of nonradioactive labeling.