ACID-POLYACRYLAMIDE GEL-ELECTROPHORESIS OF LYSOZYME-ESTRONE GLUCURONIDE CONJUGATES

Authors
SMALES CM ELGAR DF MOORE CH BLACKWELL LF
Citation
Cm. Smales et al., ACID-POLYACRYLAMIDE GEL-ELECTROPHORESIS OF LYSOZYME-ESTRONE GLUCURONIDE CONJUGATES, Bioconjugate chemistry, 9(6), 1998, pp. 838-841
Citations number
16
Categorie Soggetti
Chemistry Inorganic & Nuclear",Biology,"Biochemical Research Methods",Chemistry
Journal title
Bioconjugate chemistry → ACNP
ISSN journal
10431802
Volume
9
Issue
6
Year of publication
1998
Pages
838 - 841
Database
ISI
SICI code
1043-1802(1998)9:6<838:AGOLG>2.0.ZU;2-L
Abstract
Acid-polyacrylamide gel electrophoresis (acid-PACE) was used for analy sis of lysozyme-estrone glucuronide conjugates. The resolution of the system allowed the identification of individual conjugate families whi ch differed only in the position of acylation or in the number of estr one glucuronide units. Acid-PAGE was a good alternative to denaturing cation-exchange chromatography for the analysis, separation, and small -scale purification of lysozyme-estrone glucuronide conjugates. It rev ealed the true order of the relative degree of positive charge on the lysozyme-estrone glucuronide conjugates.