D. Hermsmeier et al., DIFFERENTIAL DISPLAY ANALYSIS OF THE EARLY COMPATIBLE INTERACTION BETWEEN SOYBEAN AND THE SOYBEAN CYST-NEMATODE, Molecular plant-microbe interactions, 11(12), 1998, pp. 1258-1263
The marked cellular changes during feeding site formation of the soybe
an cyst nematode (Heterodera glycines) indicate major changes in soybe
an gene expression. We used differential display of mRNA to detect hos
t gene expression changes during the early compatible interaction betw
een soybean and H, glycines, Fifteen cDNA clones corresponding to mRNA
s with different abundances in N, glycines-infected versus uninfected
roots were identified. Differential display results indicated that abu
ndances of five mRNAs increased in infected roots, whereas abundances
of 10 mRNAs decreased. Transcripts for nine of these 15 cDNAs could be
detected on RNA blots, and their hybridization signals confirmed the
differential display results for eight of these nine cDNAs, Sequence a
nalyses identified five cDNAs with decreased mRNA levels in infected r
oots as corresponding to two putative aldolase genes, a transcription-
factor TFIIA homologue, the soybean small GTP-binding protein gene sra
1, and the soybean auxin down-regulated gene ADR12. RNA blot analyses
of other auxin down-regulated genes revealed a decrease in their mRNA
abundances in H, glycines-infected roots as well.