Emv. Reiche et al., EVALUATION OF THE WESTERN-BLOT IN THE CONFIRMATORY SEROLOGIC DIAGNOSIS OF CHAGAS-DISEASE, The American journal of tropical medicine and hygiene, 59(5), 1998, pp. 750-756
Citations number
36
Categorie Soggetti
Public, Environmental & Occupation Heath","Tropical Medicine
The Western blot method, using antigens from epimastigote forms of the
Trypanosoma cruzi Y strain, was evaluated for the confirmatory diagno
sis of Chagas' disease. Serum samples were obtained from 136 chagasic
patients (Group I), 23 patients with inconclusive serologic results fo
r Chagas' disease (Group II), 53 patients with other diseases (Group I
II), and 50 healthy individuals (Group IV). The Western blot results f
or Group I gave a confirmatory diagnosis of Chagas' diseases in 118 (8
6.80%), an indeterminate pattern of reactivity in 16 (11.76%), and a n
egative pattern in only two (1.47%). Of the samples from Groups II, II
I and IV, none had a positive result in the Western blot; 60.86%, 52.8
3%, and 16.0%, respectively, showed indeterminate results; and 39.13%,
47,16% and 84,0%, respectively, showed negative results. The Western
blot method showed a sensitivity of 86.60%, a specificity of 100.0%, a
positive predictive value of 100.0%, and a negative predictive value
of 73.50%, and the concordance coefficient kappa was high (0.7789). Th
e results suggest that the previous serologic results for Chagas' dise
ase could be confirmed by Western blot for the detection of specific a
ntibodies to T. cruzi antigenic fractions, which may reduce the medica
l, legal, and social consequences of an inconclusive serologic result
for Chagas' disease and also underscore the need for additional studie
s for continued efforts in the development of an ideal standard confir
matory test for Chagas' disease.