Bg. Klupp et al., BOVINE HERPESVIRUS-1 GLYCOPROTEIN-B DOES NOT PRODUCTIVELY INTERACT WITH CELL-SURFACE HEPARAN-SULFATE IN A PSEUDORABIES VIRION BACKGROUND, Journal of virology, 71(6), 1997, pp. 4838-4841
Attachment to cell surface heparan sulfate proteoglycans is the first
step in infection by several alphaherpesviruses. This interaction is p
rimarily mediated by virion glycoprotein C (gC). In herpes simplex vir
us, in the absence of the nonessential gC, heparan sulfate binding is
effected by glycoprotein B. In contrast, gC-negative pseudorabies viru
s (PrV) infects target cells aa a heparan sulfate-independent mechanis
m, indicating that PrV virion gB does not productively interact with h
eparan sulfate. To assay whether a heterologous alphaherpesvirus gB pr
otein will confer productive heparan sulfate binding on gC-negative Pr
V, gC was deleted from an infectious PrV recombinant, PrV-9112C2, whic
h expresses bovine herpesvirus 1 (BHV-1) gB instead of PrV gB. Our dat
a show that gC-negative PrV-BHV-1 gB recombinant 9112C2-Delta gC beta
was not inhibited in infection by soluble heparin, in contrast to the
gC-positive parental strain. Similar results were obtained when wild-t
ype BHV-1 was compared with a gC-negative BHV-1 mutant. Moreover, infe
ction of cells proficient or deficient in heparan sulfate biosynthesis
occurred with equal efficiency by PrV-9112C2-Delta gC beta, whereas h
eparan sulfate-positive cells showed an approximately fivefold higher
plating efficiency than heparan sulfate-negative cells with the parent
al gC-positive virus. In summary, our data show that in a PrV gC-negat
ive virion background, BHV-1 gE is not able to mediate infection by pr
oductive interaction with heparan sulfate, and they indicate the same
lack of heparin interaction for BHV-1 gB in gC-negative BHV-1.