A. Verma et Kc. Kanwar, HUMAN SPERM MOTILITY AND LIPID-PEROXIDATION IN DIFFERENT ASCORBIC-ACID CONCENTRATIONS - AN IN-VITRO ANALYSIS, Andrologia, 30(6), 1998, pp. 325-329
Human spermatozoal motility, viability and lipid peroxidation (LPO) ha
ve been assessed in Ringer-Tyrode supplemented with different concentr
ations of ascorbic acid (AA) ranging from 50 to 4000 mu M. Ascorbic ac
id in concentrations below 1000 mu M protects spermatozoa from free ra
dical damage as evidenced from improvement in their motility and viabi
lity. Concomitantly, there is also witnessed depletion of malondialdeh
yde generation tan end product of LPO) following AA treatment. Ascorbi
c acid at 1000 mu M concentration and above, however, is not protectiv
e, as evidenced by abrupt fall in sperm motility and viability and con
comitant increase in LPO.