NERVE GROWTH-FACTOR IN NORMAL AND PSORIATIC SKIN EQUIVALENT MODELS

Citation
Nv. Konstantinova et al., NERVE GROWTH-FACTOR IN NORMAL AND PSORIATIC SKIN EQUIVALENT MODELS, Archives of dermatological research (Print), 290(11), 1998, pp. 610-614
Citations number
28
Categorie Soggetti
Dermatology & Venereal Diseases
ISSN journal
03403696
Volume
290
Issue
11
Year of publication
1998
Pages
610 - 614
Database
ISI
SICI code
0340-3696(1998)290:11<610:NGINAP>2.0.ZU;2-3
Abstract
Our objective was to determine the pattern and time course of nerve gr owth factor expression in an established skin equivalent model that we have used in the past to study wound healing and psoriasis phenotypes , Skin equivalents mere constructed in triplicate using normal neonata l foreskin keratinocytes plated on collagen gels containing fibroblast lines. These lines were derived from five specimens of psoriatic lesi ons, three specimens of normal skin from patients with psoriasis, and three specimens of eyelid skin from normal donors. Immunohistochemistr y and a monoclonal nerve growth factor-b antibody were used to determi ne the pattern of protein staining over 2 weeks, We looked at the woun d healing phenotype using the skin equivalent model for 7-14 days. Whe n keratinocytes invaginate into the dermis of skin equivalents (beginn ing at around 7 days of growth), dark staining of nerve growth factor was seen under the basal membrane zone, suggesting that nerve growth f actor serves in the development of the basal membrane zone and the epi dermis, and may influence the migration of nerves through the basal me mbrane zone into the regenerated skin.