EFFECTIVE PROCEDURES FOR THE EXTRACTION, AMPLIFICATION AND SEQUENCINGOF ODONATE DNA

Citation
Pt. Chippindale et al., EFFECTIVE PROCEDURES FOR THE EXTRACTION, AMPLIFICATION AND SEQUENCINGOF ODONATE DNA, Odonatologica, 27(4), 1998, pp. 415-424
Citations number
14
Categorie Soggetti
Entomology
Journal title
ISSN journal
03750183
Volume
27
Issue
4
Year of publication
1998
Pages
415 - 424
Database
ISI
SICI code
0375-0183(1998)27:4<415:EPFTEA>2.0.ZU;2-E
Abstract
The methods of specimen preservation, DNA extraction, DNA amplificatio n, choice of primers, and DNA sequencing are described. These are prim arily adaptations of those developed by other workers, but the Authors describe modifications that they have found to be optimal when workin g with odonates. It is likely that some of the described protocols wil l have more general applications to other arthropods as well.