ANALYSIS OF 3P ALLELIC LOSSES IN RENAL-CELL CARCINOMAS - COMPARISON WITH CYTOGENETIC RESULTS

We performed a study of loss of heterozygosity (LOH) at 3p by restrict ion fragment length polymorphism analysis in a series of 22 renal tumo rs. In 11 cases, molecular results could be compared with those of cyt ogenetic studies. The highest frequency of allelic losses at 3p nas se en in clear cell nonpapillary renal tumors, whereas none of the papill ary renal cell carcinomas showed LOH at 3p. Allelic losses on 3p were found to be independent of tumor grade or stage or both. One case anal yzed showed a discrepancy between cytogenetic and LOH studies. This tu mor displayed rearrangements of chromosome 3 and no LOH at the c-RAF-1 (close to the Von Hippel Lindau gene) locus. (C) Elsevier Science Inc ., 1998.