HIGH-SPEED SPECTRAL IMAGER FOR IMAGING TRANSIENT FLUORESCENCE PHENOMENA

We describe fluorescence spectral imaging results with the microscope computad-tomography imaging spectrometer (mu CTIS). This imaging spect rometer is capable of recording spatial and spectral data simultaneous ly. Consequently, mu CTIS can be used to image dynamic phenomena. The results presented consist of proof-of-concept imaging results with sta tic targets composed of 6-mu m fluorescing microspheres. Image data we re collected with integration times of 18 ms, comparable with video-fr ame-rate integration times. Conversion of raw data acquired by the mu CTIS to spatial and spectral data requires postprocessing. The emissio n spectra were sampled at 10-nm intervals between 420 and 710 nm. The smallest spatial sampling interval presented is 1.7 mu m. (C) 1998 Opt ical Society of America.