5E10 - A PROSTATE-SPECIFIC SURFACE-REACTIVE MONOCLONAL-ANTIBODY

We created mAb that reacted solely with prostate epithelial cells. The strategy involved immunizing mice with a mixture of six different pro static carcinoma cell lines and selecting by flow cytometry only those antibodies that bind whole cells. The primary screening was performed using a mixture of all six prostate cell lines used in immunization t ogether with six nonprostate cell lines in the same tube. Antibodies t hat gave a bimodal pattern of surface staining were selected for furth er evaluation. The most attractive clone, designated 5E10, produced Ig G1 mAb that recognized four of the six prostatic cell lines and did no t react with non-prostate tumor cell lines, peripheral blood and bone marrow mononuclear cells and endothelial and bone marrow stromal cells . 5E10 mAb reacted with both benign and malignant prostate in eight of eight histological samples and no reactivity was noted with non-prost ate normal tissues. The 5E10 antigen is a transmembrane glycoprotein w ith a molecular weight of 110 kDa and the epitope recognized by 5E10 i s extracellular. Ongoing studies are exploring the nature of this anti gen in more depth. (C) 1998 Published by Elsevier Science Ireland Ltd. All rights reserved.