METHODS OF DETECTION OF ANTHELMINTIC RESISTANCE IN NEMATODES OF DOMESTIC-ANIMALS

The aim of the paper is to review and compare the most important in vi tro and in vivo techniques used for the detection of anthelmintic resi stance in nematodes of domestic animals. The possible routine applicat ion of in vitro methods in the diagnosis of resistance in field condit ions is discussed. In each method its relative advantages and limitati ons are described. To measure relatively small differences in resistan ce between parasite strains or to quantitate the level of resistance u nder field conditions, we recommend the use of the egg hatch assay and larval development assays, which appeared to be the most suitable in vitro methods for field screening of anthelmintic resistance. Tests wo uld be carried out together with iii vivo faecal egg count reduction t est to compare and correlate the in vivo and in vitro results.