SYNTHETIC ANTIGEN FROM A PEPTIDE LIBRARY CAN BE AN EFFECTIVE POSITIVECONTROL IN IMMUNOASSAYS FOR THE DETECTION AND IDENTIFICATION OF 2 GEMINIVIRUSES

Phage-displayed peptides were selected from the Cys 1 random phage dis play peptide library that bound strongly to the monoclonal antibody (M Ab) SCR 20. The binding peptides were fused to the N-terminus of the p hage protein pVIII. Preparations of the phage were shown to be effecti ve as controls for the functionality of the SCR 20 MAb in both enzyme- linked immunosorbent assays and dot blot immunoassays. UV irradiation that eliminated phage infectivity did not greatly alter the antigenici ty. Peptides displayed on phage are quick and cheap to prepare, and pr eparations can be standardized to ensure comparability among different assays. The peptide library approach can be readily extended for use with other MAbs to obtain inexpensive and safe standardized positive c ontrol reagents for use in immunoassays to diagnose plant disease.