Eight spirostanol saponins, including four new compounds, and two know
n furostanol saponins were isolated from the fresh bulbs of Lilium can
didum. The structures of new compounds were determined to be (25R,26R)
-26-methoxyspirost-5-ene-3 beta,17 alpha-diol ranosyl-(1-->2)-O-[beta-
D-glucopyranosyl-(1-->4)]- beta-D-glucopyranoside}, (25R,26R)-26-metho
xyspirost-5-ene-3 beta, 17 alpha-diol -D-glucopyranosyl-(1-->4)]-beta-
Dglucopyranoside}, (25R,26R)-26-methoxyspirost-5-ene-3 beta, 17 alpha-
diol -L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside) and (25S)-spir
ost-5-ene-3 beta,27-diol 3-O-{O-beta-D-glucopyranosyl-(1 D-glucopyrano
syl-(1-->4)]-beta-D-glucopyranoside}, respectively, on the basis of sp
ectroscopic analysis, including two-dimensional NMR techniques, and th
e result of hydrolysis. The inhibitory activity of the isolated saponi
ns on Na+/K+ ATPase was evaluated.