AUTOMATIC SYSTEM FOR THE ASSAY OF GUANIDINO COMPOUNDS TO ASSESS UREMIC STATUS AND EFFECT OF HEMODIALYSIS

An automated HPLC system coupled with fluorometry Has established for the sensitive, rapid, and accurate assay of serum guanidines. Naturall y fluorescent materials characteristic of the sera of uremic patients (uremic fluorescences) which interfere with the assay were removed sim ultaneously with deproteinization, Application of this method revealed that the uremic patients who are capable of excreting: urine under he modialysis therapy show low serum guanidinosuccinic acid levels. The i nterval between hemodialysis sessions in one of these patients was pro longed while monitoring guanidinosuccinic acid level using the present method without any hazardous effect.