BINDING OF ENDONUCLEASE-VII TO CRUCIFORM DNA - VISUALIZATION IN THE ELECTRON-MICROSCOPE

Authors
KUPFER C LEE S KEMPER B
Citation
C. Kupfer et al., BINDING OF ENDONUCLEASE-VII TO CRUCIFORM DNA - VISUALIZATION IN THE ELECTRON-MICROSCOPE, The Journal of biological chemistry, 273(48), 1998, pp. 31637-31639
Citations number
21
Categorie Soggetti
Biology
Journal title
The Journal of biological chemistry → ACNP
ISSN journal
00219258
Volume
273
Issue
48
Year of publication
1998
Pages
31637 - 31639
Database
ISI
SICI code
0021-9258(1998)273:48<31637:BOETCD>2.0.ZU;2-A
Abstract
The binding of Holliday structure resolving endonuclease VII to crucif orm DNA was studied in the electron microscope. The protein was found to bind either to the junction or to one of the arms or an end of one of the arms of the construct. The amount of bound protein was determin ed by measuring the size of the complexes. On average, one complex con taining three dimers was found per one molecule of cruciform DNA.