GENOTYPING FOR POLYMORPHISMS IN INTERFERON-GAMMA, INTERLEUKIN-10, TRANSFORMING GROWTH-FACTOR-BETA-1 AND TUMOR-NECROSIS-FACTOR-ALPHA GENES -A TECHNICAL REPORT
C. Perrey et al., GENOTYPING FOR POLYMORPHISMS IN INTERFERON-GAMMA, INTERLEUKIN-10, TRANSFORMING GROWTH-FACTOR-BETA-1 AND TUMOR-NECROSIS-FACTOR-ALPHA GENES -A TECHNICAL REPORT, Transplant immunology, 6(3), 1998, pp. 193-197
Polymorphic Variants of cytokine genes are associated with acute and c
hronic transplant rejection. In this technical report, the methods cur
rently used in our centre to genotype individuals for interferon-gamma
, interleukin-10, transforming growth factor-beta 1 and tumour necrosi
s factor-alpha are described in detail. The DNA sequences of primers a
nd probes, and conditions for polymerase chain reactions are given, an
d the allele and genotype frequencies in our control populations are s
ummarized.