C. Pradhan et al., EFFICIENT PLANT-REGENERATION FROM CELL-SUSPENSION DERIVED CALLUS OF EAST-INDIAN ROSEWOOD (DALBERGIA LATIFOLIA ROXB.), Plant cell reports, 18(1-2), 1998, pp. 138-142
A procedure is outlined for the establishment of a proliferating cell
suspension culture of East Indian rosewood (Dalbergia latifolia Roxb.)
and efficient plant regeneration from callus derived from such cultur
es. Callus was induced from hypocotyl segments derived from 1-week-old
axenic seedlings on Murashige and Skoog (1962) medium (MS) containing
10.8 mu M naphthaleneacetic acid (NAA) and 2.2 mu M benzyladenine (BA
). Calli were increased by subculturing on MS supplemented with same g
rowth regulators and 10% coconut water (CW). Friable calli were used t
o initiate cell suspension cultures. Optimum cell proliferation occurr
ed in MS containing 10.8 mu M NAA, 2.2 mu M BA and 10% CW, using an in
itial inoculum cell density of 2%. Cell clumps composed of 20-25 cells
harvested from suspension cultures at the exponential growth phase re
adily formed callus within 3 weeks following plating on the semi-solid
MS as above. High-frequency shoot-bud differentiation was induced in
these calli on MS containing 2.7 mu M NAA and 13.3 mu M BA. The regene
ration frequency declined at higher BA concentrations. The organogenic
potential of the cell suspensions was influenced by the age of the cu
lture. Regenerated shoots were rooted on half-strength MS containing 5
.7 mu M indole-3-acetic acid, 4.9 mu M indole-3-butyric acid and 5.3 m
u M indole-3-propionic acid. The plantlets were acclimatized and estab
lished in soil.