Bv. Le et al., RAPID REGENERATION OF WHOLE PLANTS IN LARGE CRABGRASS (DIGITARIA SANGUINALIS L.) USING THIN CELL LAYER CULTURE, Plant cell reports, 18(1-2), 1998, pp. 166-172
A method was designed to produce rapidly (10-14 days) and directly (wi
thout intermediate callus) whole plants of Digitaria sanguinalis with
a high yield without subculture. These plants developed from new struc
tures, designated ''pseudo-embryogenic structures'', initiated only 1
week after the culture of tranverse thin cell layers (tTCLs), i.e., th
in stem sections, on a Murashige and Skoog medium containing 3% sucros
e and a combination of a low concentration of 2,4-dichlorophenoxyaceti
c acid (1 mu M) and a high concentration of 6-benzylaminopurine (10 mu
M). The fresh weight of plants regenerated per tTCL on gelrite was 6
times higher than with agar and 30 times higher than with agarose.