2-CHLORO-1,4-DIMETHOXYBENZENE AS A MEDIATOR OF LIGNIN PEROXIDASE-CATALYZED OXIDATIONS

Poly R-478, 4-methoxymandelic acid and oxalic acid sere oxidized by li gnin peroxidase (LIP) in the presence of the fungal metabolite 2-chlor o-1,4-dimethoxybenzene (2Cl-14DMB), whereas no oxidation occurred in t he absence of 2Cl-14DMB. These substrates clearly inhibited the consum ption of 2Cl-14DMB and the formation of 2-chloro-1,4-benzoquinone from 2Cl-14DMB by LIP. The results suggest that 2Cl-14DMB can replace the function of veratryl alcohol as a redox mediator in lignin peroxidase catalyzed oxidations. (C) 1998 Federation of European Biochemical Soci eties.