ANALYSIS OF REVERSE-PHASE CHROMATOGRAPHIC SEPARATED BACILLUS-SUBTILISMEMBRANE-PROTEINS

Authors
BHARDWAJ S DAY RA
Citation
S. Bhardwaj et Ra. Day, ANALYSIS OF REVERSE-PHASE CHROMATOGRAPHIC SEPARATED BACILLUS-SUBTILISMEMBRANE-PROTEINS, Analytical letters, 31(15), 1998, pp. 2625-2634
Citations number
19
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
31
Issue
15
Year of publication
1998
Pages
2625 - 2634
Database
ISI
SICI code
0003-2719(1998)31:15<2625:AORCSB>2.0.ZU;2-9
Abstract
B. subtilis membrane proteins were separated by reverse phase chromato graphy using a simple solvent system of acetonitrile/water. The isolat ed proteins were further characterized by a 'MS-FIT' peptide mass fing erprinting program. The experimental scheme for separation and identif ication of membrane proteins could also be modified to detect protein- protein interactions. The method has been proven useful for rapid isol ation, purification, and characterization of hydrophobic membrane prot eins.