A simple and efficient HPLC analysis method has been developed to simu
ltaneously analyse complicated multi-vitamin preparations, including v
itamin B complexes, vitamin C, erotic acid, methionine, calcium pantot
henate, nicotinamide, biotin, folic acid and rutin, without using expe
nsive ion pairing reagent. Diode array detector and adjustment of the
mobile phase to pH 5.6 are required in the study. This method has been
validated with good linearity, reproducibility, recovery and accuracy
for analysis of commercial multi-vitamin products.